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CACNA1D Polyclonal antibody

CACNA1D Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22276-1-AP

Synonyms

CACH3, CACN4, CACNA1D, CACNL1A2, Cav1.3, CCHL1A2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CACNA1D Polyclonal antibody (22276-1-AP)

    WB analysis of HEK-293 using 22276-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22276-1-AP (CACNA1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using CACNA1D Polyclonal antibody (22276-1-AP)

    IHC staining of human brain using 22276-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22276-1-AP (CACNA1D Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CACNA1D Polyclonal antibody (22276-1-AP)

    IHC staining of human brain using 22276-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22276-1-AP (CACNA1D Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CACNA1D Polyclonal antibody (22276-1-AP)

    IF Staining of SH-SY5Y using 22276-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 22276-1-AP (CACNA1D antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22276-1-AP targets CACNA1D in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name calcium channel, voltage-dependent, L type, alpha 1D subunit
Calculated Molecular Weight 248 kDa
Observed Molecular Weight 200-250 kDa
GenBank Accession NumberNM_000720
Gene Symbol CACNA1D
Gene ID (NCBI) 776
RRIDAB_2879057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CACNA1D antibody 22276-1-APDownload protocol
IHC protocol for CACNA1D antibody 22276-1-APDownload protocol
IF protocol for CACNA1D antibody 22276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

David (Verified Customer) (10-12-2022)

AB works well in rat aortic tissue. 1:500 dilution over night.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: rat aortic tissue