CACNA2D1 Polyclonal antibody
CACNA2D1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 27453-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse brain tissue, pig brain tissue, rat brain tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 4 publications below |
Product Information
27453-1-AP targets CACNA2D1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
| Tested Reactivity | human, mouse, pig, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CACNA2D1 fusion protein Ag26190 |
| Full Name | calcium channel, voltage-dependent, alpha 2/delta subunit 1 |
| Calculated Molecular Weight | 125 kDa |
| Observed Molecular Weight | 125 kDa |
| GenBank Accession Number | NM_000722 |
| Gene Symbol | CACNA2D1 |
| Gene ID (NCBI) | 781 |
| RRID | AB_2880874 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CACNA2D1 antibody 27453-1-AP | Download protocol |
| IHC protocol for CACNA2D1 antibody 27453-1-AP | Download protocol |
| IF protocol for CACNA2D1 antibody 27453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Behav Immun Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain. | ||
Heliyon Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1 | ||
Cells Calcium-Related Genes Predicting Outcomes and Serving as Therapeutic Targets in Endometrial Cancer | ||
Ecotoxicol Environ Saf Cigarette smoke triggers calcium overload in mouse hippocampal neurons via the ΔFOSB-CACNA2D1 axis to impair cognitive performance |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christian (Verified Customer) (11-19-2018) | 1:50 - labelled neurons of interest1:200 - labelled 3-4 neurons of interest1:1000 - labelled 1-2 neurons of interest
 |
