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  • Featured Product
  • KD/KO Validated

CALCOCO1 Polyclonal antibody

CALCOCO1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19843-1-AP

Synonyms

CALCOCO1, calphoglin, Cocoa, KIAA1536, PP13275, Sarcoma antigen NY SAR 3, UNQ2436/PRO4996

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using CALCOCO1 Polyclonal antibody (19843-1-AP)

    WB analysis of HT-1080 using 19843-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19843-1-AP (CALCOCO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CALCOCO1 Polyclonal antibody (19843-1-AP)

    WB analysis of MCF-7 using 19843-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19843-1-AP (CALCOCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CALCOCO1 Polyclonal antibody (19843-1-AP)

    IHC staining of human testis using 19843-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CALCOCO1 Polyclonal antibody (19843-1-AP)

    IHC staining of human testis using 19843-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CALCOCO1 Polyclonal antibody (19843-1-AP)

    IHC staining of human kidney using 19843-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CALCOCO1 Polyclonal antibody (19843-1-AP)

    IHC staining of human kidney using 19843-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19843-1-AP (CALCOCO1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CALCOCO1 Polyclonal antibody (19843-1-AP)

    IF Staining of HeLa using 19843-1-AP

    Immunofluorescent analysis of Hela cells, using CALCOCO1 antibody 19843-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHT-1080 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19843-1-AP is CALCOCO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALCOCO1 fusion protein Ag13876
Full Name calcium binding and coiled-coil domain 1
Calculated Molecular Weight 691 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC003177
Gene Symbol CALCOCO1
Gene ID (NCBI) 57658
RRIDAB_10637265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CALCOCO1 antibody 19843-1-APDownload protocol
IHC protocol for CALCOCO1 antibody 19843-1-APDownload protocol
IF protocol for CALCOCO1 antibody 19843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Elife

Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.

Authors - Junghyun Lim
  • KO Validated
View Article
mouseWB

Cell Signal

Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.

Authors - Lisa M Cooper
View Article
mouseWB

Nat Commun

Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer

Authors - Lucia Taraborrelli
View Article