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Calmodulin1/2/3 Polyclonal antibody

Calmodulin1/2/3 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 10112-1-AP

Synonyms

CALM, CALM1, CALM2, CALM3, CALML2, Calmodulin, CaM, CAM1, CAM2, CAM3, CAMB, CAMC, CAMIII, PHKD, PHKD3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Calmodulin1/2/3 Polyclonal antibody (10112-1-AP)

    IHC staining of human lung cancer using 10112-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10112-1-AP (CALM3 antibody) at dilution of 1:50 (under 10x lens).

  • Flow cytometry (FC) experiment of MCF-7 cells using Calmodulin1/2/3 Polyclonal antibody (10112-1-AP)

    FC experiment of MCF-7 using 10112-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug CALM3 antibody (10112-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10112-1-AP targets Calmodulin1/2/3 in IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calmodulin1/2/3 fusion protein Ag0146
Full Name calmodulin 3 (phosphorylase kinase, delta)
Calculated Molecular Weight 17 kDa
GenBank Accession NumberBC005137
Gene Symbol CALM3
Gene ID (NCBI) 808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for Calmodulin1/2/3 antibody 10112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols