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CALML3 Polyclonal antibody

CALML3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17275-1-AP

Synonyms

CALML3, calmodulin like 3, Calmodulin like protein 3, CaM like protein, CLP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CALML3 Polyclonal antibody (17275-1-AP)

    WB analysis using 17275-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17275-1-AP (CALML3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using CALML3 Polyclonal antibody (17275-1-AP)

    IP experiment of A431 using 17275-1-AP

    IP result of anti-CALML3 (IP:17275-1-AP, 4ug; Detection:17275-1-AP 1:300) with A431 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CALML3 Polyclonal antibody (17275-1-AP)

    IHC staining of human liver cancer using 17275-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17275-1-AP (CALML3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CALML3 Polyclonal antibody (17275-1-AP)

    IHC staining of human liver cancer using 17275-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17275-1-AP (CALML3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CALML3 Polyclonal antibody (17275-1-AP)

    IF Staining of A431 using 17275-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CALML3 antibody (17275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17275-1-AP targets CALML3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALML3 fusion protein Ag11037
Full Name calmodulin-like 3
Calculated Molecular Weight 149 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC031889
Gene Symbol CALML3
Gene ID (NCBI) 810
RRIDAB_2878374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CALML3 antibody 17275-1-APDownload protocol
IHC protocol for CALML3 antibody 17275-1-APDownload protocol
IF protocol for CALML3 antibody 17275-1-APDownload protocol
IP protocol for CALML3 antibody 17275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols