CAMK1 Polyclonal antibody

CAMK1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24312-1-AP

Synonyms

CaM KI, CaM kinase I, CaM kinase I alpha, CAMK1, CAMKI, CaMKI alpha



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24312-1-AP is CAMK1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAMK1 fusion protein Ag19449
Full Name calcium/calmodulin-dependent protein kinase I
Calculated Molecular Weight 370 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC106755
Gene Symbol CAMK1
Gene ID (NCBI) 8536
RRIDAB_2879489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CAMK1 antibody 24312-1-APDownload protocol
IHC protocol for CAMK1 antibody 24312-1-APDownload protocol
IF protocol for CAMK1 antibody 24312-1-APDownload protocol
IP protocol for CAMK1 antibody 24312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Res Int

Effect of Lentivirus-Mediated miR-499a-3p on Human Umbilical Vein Endothelial Cells.

Authors - Huilei Zheng
mouseIHC

Biochim Biophys Acta Mol Basis Dis

IGF2BP3-stabilized CAMK1 regulates the mitochondrial dynamics of renal tubule to alleviate diabetic nephropathy

Authors - Du Yuan