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  • KD/KO Validated

CAPZA2 Polyclonal antibody

CAPZA2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15948-1-AP

Synonyms

CAPPA2, CAPZ, CapZ alpha 2, CAPZA2



Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, MCF-7 cells, mouse heart tissue, mouse liver tissue, mouse ovary tissue, mouse skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15948-1-AP targets CAPZA2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPZA2 fusion protein Ag8544
Full Name capping protein (actin filament) muscle Z-line, alpha 2
Calculated Molecular Weight 286 aa, 33 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberBC005338
Gene Symbol CAPZA2
Gene ID (NCBI) 830
RRIDAB_2070148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CAPZA2 antibody 15948-1-APDownload protocol
IHC protocol for CAPZA2 antibody 15948-1-APDownload protocol
IP protocol for CAPZA2 antibody 15948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Elife

HIPK4 is essential for murine spermiogenesis.

Authors - J Aaron Crapster
mouseWB

Oncotarget

Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer.

Authors - Mi Jeong Kwon
  • KD Validated
humanWB

Biochem J

Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation.

Authors - João D Santos