CARD6 Polyclonal antibody

CARD6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 18029-1-AP

Synonyms

CARD6, CINCIN1



Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18029-1-AP targets CARD6 in WB, IHC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARD6 fusion protein Ag12595
Full Name caspase recruitment domain family, member 6
Calculated Molecular Weight 1037 aa, 116 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC093825
Gene Symbol CARD6
Gene ID (NCBI) 84674
RRIDAB_2290686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The caspase recruitment domain (CARD) is a homotypic protein-protein interaction module that links components of signal transduction pathways implicated in the regulation of apoptosis or adaptive or innate immunity. Although much progress has been made in assigning precise roles to most CARD-containing proteins, the functions of the 1,037-amino-acid (aa) human and 1,175-aa mouse CARD6 proteins are still unknown. CARD6 has a unique structure in that it contains the CARD at the N terminus, a glutamic acid-rich region following the CARD, and a proline-rich region at the C terminus. CARD6 also harbors a 350-aa region with similarity to upregulated gene 4 (URG4), a protein that is induced in response to hepatitis Bx antigen overexpression and exerts a positive effect on proliferation. Both CARD6 and URG4 share structural features with members of the multifaceted, IFN-inducible GTPase (IFNiGTPase) superfamily, which contains some of the proteins most abundantly induced during cell-autonomous immune responses. The calcualted molecular weight of CARD is 116 kDa, but modified CARD6 is about 130 kDa. (PMID: 18160713)

Protocols

Product Specific Protocols
WB protocol for CARD6 antibody 18029-1-APDownload protocol
IHC protocol for CARD6 antibody 18029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cell Mol Med

Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Authors - Yong Zhang