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CBP20 Polyclonal antibody

CBP20 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11950-1-AP

Synonyms

CBC2, CBP20, NCBP 20 kDa subunit, NCBP interacting protein 1, NCBP2, NIP1, PIG55

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using CBP20 Polyclonal antibody (11950-1-AP)

    WB analysis of human heart using 11950-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11950-1-AP (CBP20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CBP20 Polyclonal antibody (11950-1-AP)

    IHC staining of mouse brain using 11950-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11950-1-AP (CBP20 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CBP20 Polyclonal antibody (11950-1-AP)

    IHC staining of mouse brain using 11950-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11950-1-AP (CBP20 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBP20 Polyclonal antibody (11950-1-AP)

    IF Staining of HeLa using 11950-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11950-1-AP (CBP20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11950-1-AP targets CBP20 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBP20 fusion protein Ag2550
Full Name nuclear cap binding protein subunit 2, 20kDa
Calculated Molecular Weight 156 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC001255
Gene Symbol NCBP2
Gene ID (NCBI) 22916
RRIDAB_2150142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CBP20 antibody 11950-1-APDownload protocol
IHC protocol for CBP20 antibody 11950-1-APDownload protocol
IF protocol for CBP20 antibody 11950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
View Article
humanWB,IHC

Eur J Med Res

N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma

Authors - Aierpati Maimaiti
View Article