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  • Featured Product
  • KD/KO Validated

CBX5 Polyclonal antibody

CBX5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 11831-1-AP

Synonyms

Antigen p25, CBX5, Chromobox protein homolog 5, HP1, HP1 alpha, HP1A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CBX5 Polyclonal antibody (11831-1-AP)

    WB analysis of HeLa using 11831-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CBX5 Polyclonal antibody (11831-1-AP)

    WB analysis of K-562 using 11831-1-AP

    WB result of CBX5 antibody (11831-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX5 transfected K-562 cells.

  • Western Blot (WB) analysis of human kidney tissue using CBX5 Polyclonal antibody (11831-1-AP)

    WB analysis of human kidney using 11831-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CBX5 Polyclonal antibody (11831-1-AP)

    WB analysis of HEK-293 using 11831-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CBX5 Polyclonal antibody (11831-1-AP)

    WB analysis of K-562 using 11831-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CBX5 Polyclonal antibody (11831-1-AP)

    WB analysis of A431 using 11831-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CBX5 Polyclonal antibody (11831-1-AP)

    WB analysis of MCF-7 using 11831-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CBX5 Polyclonal antibody (11831-1-AP)

    WB analysis of HEK-293 using 11831-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CBX5 Polyclonal antibody (11831-1-AP)

    WB analysis of MCF-7 using 11831-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CBX5 Polyclonal antibody (11831-1-AP)

    IP experiment of HEK-293 using 11831-1-AP

    IP result of anti-CBX5 (IP:11831-1-AP, 4ug; Detection:11831-1-AP 1:500) with HEK-293 cells lysate 1120ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CBX5 Polyclonal antibody (11831-1-AP)

    IHC staining of human lung cancer using 11831-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11831-1-AP (CBX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CBX5 Polyclonal antibody (11831-1-AP)

    IHC staining of human lung cancer using 11831-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11831-1-AP (CBX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, human kidney tissue, K-562 cells, A431 cells, MCF-7 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11831-1-AP targets CBX5 in WB, IP, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBX5 fusion protein Ag2403
Full Name chromobox homolog 5 (HP1 alpha homolog, Drosophila)
Calculated Molecular Weight 191 aa, 22 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC006821
Gene Symbol CBX5
Gene ID (NCBI) 23468
RRIDAB_2071356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromobox protein homolog 5 (CBX5), also named heterochromatin protein 1 alpha (HP1a), is a highly conserved nonhistone protein involved in heterochromatin formation and gene silencing in different species including humans.HP1a is a Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). It may interact with lamin-Breceptor. HP1a is involved in the formation of functional kinetochore through interaction with MIS12complex proteins. Phosphorylation of HP1 and LBR during interphase mitosis may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.The HP1a was expressed in nucleus and associates specifically with chromatin during metaphase and anaphase.Recent studies have shown that HP1a is present at many euchromatic sites and positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila (19798443). This antibody is a rabbit polyclonal antibody raised against a full-length human HP1A protein ,and can reacts with the 28kd HP1A protein.

Protocols

Product Specific Protocols
WB protocol for CBX5 antibody 11831-1-APDownload protocol
IHC protocol for CBX5 antibody 11831-1-APDownload protocol
IP protocol for CBX5 antibody 11831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

J Exp Med

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

Authors - Longfei Ma
View Article
mouseWB

Nat Commun

The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.

Authors - Guang Yang
View Article
humanWB,chIP

Cancer Res

ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.

Authors - Lili Zhang
View Article
humanWB

Asian Pac J Cancer Prev

Triptolide Promotes Senescence of Prostate Cancer Cells Through Histone Methylation and Heterochromatin Formation

Authors - Ousman Tamgue
View Article
humanWB

Microbiol Spectr

Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis

Authors - Ruyi Fan
  • KD Validated
View Article
humanWB,IP

Oncogene

ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression

Authors - Lifang Li
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

WEI (Verified Customer) (05-11-2022)

Specific bands at predicted size.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse Heart