• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CCDC105 Polyclonal antibody

CCDC105 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24026-1-AP

Synonyms

CCDC105

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of DU 145 cells using CCDC105 Polyclonal antibody (24026-1-AP)

    WB analysis of DU 145 using 24026-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CCDC105 Polyclonal antibody (24026-1-AP)

    WB analysis of HeLa using 24026-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CCDC105 Polyclonal antibody (24026-1-AP)

    WB analysis of SH-SY5Y using 24026-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC105 Polyclonal antibody (24026-1-AP)

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC105 Polyclonal antibody (24026-1-AP)

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC105 Polyclonal antibody (24026-1-AP)

    IF Staining of HeLa using 24026-1-AP

    Immunofluorescent analysis of HeLa cells using 24026-1-AP (CCDC105 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CCDC105 Polyclonal antibody (24026-1-AP)

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CCDC105 Polyclonal antibody (24026-1-AP)

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24026-1-AP targets CCDC105 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC105 fusion protein Ag21208
Full Name coiled-coil domain containing 105
Calculated Molecular Weight 499 aa, 57 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC035678
Gene Symbol CCDC105
Gene ID (NCBI) 126402
RRIDAB_2879406
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCDC105 antibody 24026-1-APDownload protocol
IHC protocol for CCDC105 antibody 24026-1-APDownload protocol
IF protocol for CCDC105 antibody 24026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols