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CCDC28A Polyclonal antibody

CCDC28A Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14807-1-AP

Synonyms

C6orf80, CCDC28A, CCRL1AP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis using 14807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis of HeLa using 14807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis of mouse thymus using 14807-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCDC28A Polyclonal antibody (14807-1-AP)

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (14807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCDC28A Polyclonal antibody (14807-1-AP)

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CCDC28A antibody 14807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA375 cells, HeLa cells, mouse thymus tissue, Jurkat cells, MCF-7 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14807-1-AP targets CCDC28A in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC28A fusion protein Ag6529
Full Name coiled-coil domain containing 28A
Calculated Molecular Weight 30 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC000758
Gene Symbol CCDC28A
Gene ID (NCBI) 25901
RRIDAB_2072069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCDC28A antibody 14807-1-APDownload protocol
IF protocol for CCDC28A antibody 14807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols