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  • Featured Product
  • KD/KO Validated

TACO1/CCDC44 Polyclonal antibody

TACO1/CCDC44 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21147-1-AP

Synonyms

CCDC44, TACO1, TACO1/CCDC44

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TACO1/CCDC44 Polyclonal antibody (21147-1-AP)

    WB analysis of HeLa using 21147-1-AP

    WB result of TACO1/CCDC44 antibody (21147-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TACO1/CCDC44 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using TACO1/CCDC44 Polyclonal antibody (21147-1-AP)

    WB analysis using 21147-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using TACO1/CCDC44 Polyclonal antibody (21147-1-AP)

    IP experiment of mouse liver using 21147-1-AP

    IP result of anti-TACO1/CCDC44 (IP:21147-1-AP, 4ug; Detection:21147-1-AP 1:600) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TACO1/CCDC44 Polyclonal antibody (21147-1-AP)

    IHC staining of human kidney using 21147-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TACO1/CCDC44 Polyclonal antibody (21147-1-AP)

    IHC staining of human kidney using 21147-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TACO1/CCDC44 Polyclonal antibody (21147-1-AP)

    IHC staining of human brain using 21147-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TACO1/CCDC44 Polyclonal antibody (21147-1-AP)

    IHC staining of human brain using 21147-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21147-1-AP (TACO1/CCDC44 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse heart, rat heart
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21147-1-AP is TACO1/CCDC44 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TACO1/CCDC44 fusion protein Ag15688
Full Name coiled-coil domain containing 44
Calculated Molecular Weight 297 aa, 32 kDa
Observed Molecular Weight 30-32 kDa
GenBank Accession NumberBC007744
Gene Symbol CCDC44
Gene ID (NCBI) 51204
RRIDAB_10732604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TACO1 (translational activator of cytochrome oxidase subunit I) is required for COXI translation. Mutations in TACO1 cause Leigh syndrome (LS) and cytochrome c oxidase deficiency. (27319982)

Protocols

Product Specific Protocols
WB protocol for TACO1/CCDC44 antibody 21147-1-APDownload protocol
IHC protocol for TACO1/CCDC44 antibody 21147-1-APDownload protocol
IP protocol for TACO1/CCDC44 antibody 21147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Authors - Xiaorong Zhang
View Article
mouseWB

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler
View Article
mouseWB

Nat Commun

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Authors - Tara R Richman
  • KO Validated
View Article
mouseWB

EMBO J

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Authors - Nicola Ferreira
View Article
humanWB,IP

Free Radic Biol Med

PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis

Authors - Qiong Sun
View Article
mouseWB

Biochem Biophys Res Commun

Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.

Authors - Takumi Yokokawa
View Article