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- KD/KO Validated
CEP112 Polyclonal antibody
CEP112 Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, Canine
Applications
WB, IF,ELISA
Conjugate
Unconjugated
Cat no : 24928-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, mouse testis tissue |
| Positive IF detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 3 publications below |
Product Information
The immunogen of 24928-1-AP is CEP112 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, Canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CEP112 fusion protein Ag21586 |
| Full Name | coiled-coil domain containing 46 |
| Calculated Molecular Weight | 955 aa, 113 kDa |
| Observed Molecular Weight | 110-113 kDa |
| GenBank Accession Number | BC131805 |
| Gene Symbol | CCDC46 |
| Gene ID (NCBI) | 201134 |
| RRID | AB_2879802 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP112 antibody 24928-1-AP | Download protocol |
| IF protocol for CEP112 antibody 24928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.
| ||
PLoS Biol Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins. | ||
Clin Genet Loss-of-function mutations in centrosomal protein 112 is associated with human acephalic spermatozoa phenotype. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jay (Verified Customer) (11-08-2019) | Human RPE1 cell line with Methanol fixationImmunostaining result shows that CEP112 localizes to centrosome.(Red;Cep112, Green; Cep164 for Centrosome marker, Blue;DAPI for Nucleus).
 |
