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CWC25 Polyclonal antibody

CWC25 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24416-1-AP

Synonyms

CCDC49, CWC25

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CWC25 Polyclonal antibody (24416-1-AP)

    WB analysis of HeLa using 24416-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24416-1-AP (CWC25 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human testis using 24416-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24416-1-AP (CWC25 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human cervical cancer using 24416-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human cervical cancer using 24416-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24416-1-AP (CWC25 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CWC25 Polyclonal antibody (24416-1-AP)

    IHC staining of human testis using 24416-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24416-1-AP (CWC25 antibody at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CWC25 Polyclonal antibody (24416-1-AP)

    IF Staining of HepG2 using 24416-1-AP

    Immunofluorescent analysis of HepG2 cells using 24416-1-AP (CWC25 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24416-1-AP is CWC25 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CWC25 fusion protein Ag17037
Full Name coiled-coil domain containing 49
Calculated Molecular Weight 425 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC008833
Gene Symbol CCDC49
Gene ID (NCBI) 54883
RRIDAB_2879535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CWC25 antibody 24416-1-APDownload protocol
IHC protocol for CWC25 antibody 24416-1-APDownload protocol
IF protocol for CWC25 antibody 24416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols