• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HAUS1 Polyclonal antibody

HAUS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11094-2-AP

Synonyms

CCDC5, Enhancer of invasion cluster, HAUS1, HEI C, HEIC, HsT1461

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human skeletal muscle tissue using HAUS1 Polyclonal antibody (11094-2-AP)

    WB analysis of human skeletal muscle using 11094-2-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HAUS1 Polyclonal antibody (11094-2-AP)

    WB analysis using 11094-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HAUS1 Polyclonal antibody (11094-2-AP)

    WB analysis of HeLa using 11094-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HAUS1 Polyclonal antibody (11094-2-AP)

    IP experiment of HeLa using 11094-2-AP

    IP result of anti-HAUS1 (IP:11094-2-AP, 4ug; Detection:11094-2-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HAUS1 Polyclonal antibody (11094-2-AP)

    IHC staining of human pancreas cancer using 11094-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HAUS1 Polyclonal antibody (11094-2-AP)

    IHC staining of human pancreas cancer using 11094-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HL-60 cells, HeLa cells, MOLT-4 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11094-2-AP targets HAUS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAUS1 fusion protein Ag1569
Full Name coiled-coil domain containing 5 (spindle associated)
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC014003
Gene Symbol CCDC5
Gene ID (NCBI) 115106
RRIDAB_614986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAUS1 is a subunit of the human augmin complex that is involved in microtubule generation and mitotic spindle formation (PMID:27036161). In addition, HAUS1 may play a major role in centrosome integrity and completion of cytokinesis. The depletion of HAUS1 in cells decreases the G2/M cell compartment and induces apoptosis. High HAUS1 expression was associated with several tumour-infiltrating immune cells such as Th2 cells, macrophages, and activated dendritic cells (PMID:35865089). 11094-2-AP can detect the 32 kDa band, which is consistent with the calculated MW.

Protocols

Product Specific Protocols
WB protocol for HAUS1 antibody 11094-2-APDownload protocol
IHC protocol for HAUS1 antibody 11094-2-APDownload protocol
IP protocol for HAUS1 antibody 11094-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article
mouseWB

Nucleic Acids Res

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity

Authors - Dan Li
View Article