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CCDC53 Polyclonal antibody

CCDC53 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24445-1-AP

Synonyms

CCDC53, CGI 116, WASH complex subunit CCDC53, WASHC3, x0009

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human placenta tissue using CCDC53 Polyclonal antibody (24445-1-AP)

    IHC staining of human placenta using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CCDC53 Polyclonal antibody (24445-1-AP)

    IHC staining of human placenta using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CCDC53 Polyclonal antibody (24445-1-AP)

    IHC staining of human liver using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using CCDC53 Polyclonal antibody (24445-1-AP)

    IHC staining of human liver using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CCDC53 Polyclonal antibody (24445-1-AP)

    IHC staining of human breast cancer using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CCDC53 Polyclonal antibody (24445-1-AP)

    IHC staining of human breast cancer using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.

Tested Applications

Positive IHC detected inhuman placenta tissue, human breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

24445-1-AP targets CCDC53 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC53 fusion protein Ag19831
Full Name coiled-coil domain containing 53
Calculated Molecular Weight 194 aa, 21 kDa
GenBank Accession NumberBC010889
Gene Symbol CCDC53
Gene ID (NCBI) 51019
RRIDAB_2879551
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CCDC53 antibody 24445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Architecture of the human interactome defines protein communities and disease networks.

Authors - Edward L Huttlin
View Article
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
View Article