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CCDC68 Polyclonal antibody

CCDC68 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26301-1-AP

Synonyms

CCDC68, CTCL associated antigen se57 1, SE57 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A431 cells using CCDC68 Polyclonal antibody (26301-1-AP)

    WB analysis of A431 using 26301-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 26301-1-AP (CCDC68 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CCDC68 Polyclonal antibody (26301-1-AP)

    WB analysis of SH-SY5Y using 26301-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26301-1-AP (CCDC68 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC68 Polyclonal antibody (26301-1-AP)

    IHC staining of human testis using 26301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26301-1-AP (CCDC68 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC68 Polyclonal antibody (26301-1-AP)

    IHC staining of human testis using 26301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26301-1-AP (CCDC68 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CCDC68 Polyclonal antibody (26301-1-AP)

    IHC staining of human colon using 26301-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26301-1-AP (CCDC68 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CCDC68 Polyclonal antibody (26301-1-AP)

    IHC staining of human colon using 26301-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26301-1-AP (CCDC68 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 26301-1-AP is CCDC68 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC68 fusion protein Ag23622
Full Name coiled-coil domain containing 68
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC029508
Gene Symbol CCDC68
Gene ID (NCBI) 80323
RRIDAB_2880470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coiled-coil domain containing 68 (CCDC68) demonstrates a tumor-suppressive role in pancreatic ductal adenocarcinoma. (PMID: 25381825)

Protocols

Product Specific Protocols
WB protocol for CCDC68 antibody 26301-1-APDownload protocol
IHC protocol for CCDC68 antibody 26301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Elife

Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages.

Authors - Weng Man Chong
View Article