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CCDC72 Polyclonal antibody

CCDC72 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20393-1-AP

Synonyms

CCDC72, HSPC016, HSPC330, TMA7

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CCDC72 Polyclonal antibody (20393-1-AP)

    WB analysis of A549 using 20393-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CCDC72 Polyclonal antibody (20393-1-AP)

    WB analysis of MCF-7 using 20393-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CCDC72 Polyclonal antibody (20393-1-AP)

    WB analysis of HEK-293 using 20393-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using CCDC72 Polyclonal antibody (20393-1-AP)

    IP experiment of A549 using 20393-1-AP

    IP result of anti-CCDC72 (IP:20393-1-AP, 4ug; Detection:20393-1-AP 1:500) with A549 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human skin tissue using CCDC72 Polyclonal antibody (20393-1-AP)

    IHC staining of human skin using 20393-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CCDC72 Polyclonal antibody (20393-1-AP)

    IHC staining of human skin using 20393-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CCDC72 Polyclonal antibody (20393-1-AP)

    IF Staining of A549 using 20393-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20393-1-AP is CCDC72 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC72 fusion protein Ag13792
Full Name coiled-coil domain containing 72
Calculated Molecular Weight 64 kDa, 7 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC001102
Gene Symbol CCDC72
Gene ID (NCBI) 51372
RRIDAB_10667404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC72, also named as TMA7, is a novel modulator for hair follicle development. CCDC72 has the promoter activity and regulation of gene expression.

Protocols

Product Specific Protocols
WB protocol for CCDC72 antibody 20393-1-APDownload protocol
IHC protocol for CCDC72 antibody 20393-1-APDownload protocol
IF protocol for CCDC72 antibody 20393-1-APDownload protocol
IP protocol for CCDC72 antibody 20393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Biol Sci

IGF2BP3 Regulates TMA7-mediated Autophagy and Cisplatin Resistance in Laryngeal Cancer via m6A RNA Methylation

Authors - Like Yang
View Article
mouseWB

bioRxiv

Genetic deficiency of ribosomal rescue factor HBS1L causes retinal dystrophy associated with Pelota and EDF1 depletion

Authors - Shiyu Luo
View Article