CCL24/Eotaxin 2 Polyclonal antibody

CCL24/Eotaxin 2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 22306-1-AP

Synonyms

C C motif chemokine 24, CCL24, CK beta 6, Ckb 6, Eotaxin 2, MPIF 2, MPIF2, SCYA24, Small inducible cytokine A24



Tested Applications

Positive WB detected inmouse thymus tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

22306-1-AP targets CCL24/Eotaxin 2 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name chemokine (C-C motif) ligand 24
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberNM_002991
Gene Symbol CCL24
Gene ID (NCBI) 6369
RRIDAB_2879068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chemokine CCL24 is the second member of eotaxins, a group of eosinophils' selectively chemoattractants. CCL24 is constitutively expressed in fibroblasts, epithelial cells, macrophages. CCL24 has only one receptor CCR3, which mainly presents on eosinophils and was also found on basophils, monocytes, Th2 lymphocytes, epithelial cells and airway smooth muscles. CCL24 displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes.

Protocols

Product Specific Protocols
WB protocol for CCL24/Eotaxin 2 antibody 22306-1-APDownload protocol
IHC protocol for CCL24/Eotaxin 2 antibody 22306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dis Markers

CCL24 Protects Renal Function by Controlling Inflammation in Podocytes.

Authors - Youdi Wang