• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CCR6 Monoclonal antibody

CCR6 Monoclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C11A9

Cat no : 66801-1-Ig

Synonyms

BN 1, C C chemokine receptor type 6, C C CKR 6, CC CKR 6, CCR 6, CCR6, CD196, Chemokine receptor like 3, CKR L3, CKR6, CKRL3, CMKBR6, DCR2, DRY 6, DRY6, G protein coupled receptor 29, GPR CY4, GPR29, GPRCY4, LARC receptor, STRL22

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CCR6 Monoclonal antibody (66801-1-Ig)

    IHC staining of human tonsillitis using 66801-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CCR6 Monoclonal antibody (66801-1-Ig)

    IHC staining of human tonsillitis using 66801-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CCR6 Monoclonal antibody (66801-1-Ig)

    IF Staining of Jurkat using 66801-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CCR6 Monoclonal antibody (66801-1-Ig)

    IF Staining of human tonsillitis using 66801-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using CCR6 Monoclonal antibody (66801-1-Ig)

    FC experiment of Jurkat using 66801-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CCR6 (66801-1-Ig, Clone:1C11A9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells, human tonsillitis tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66801-1-Ig targets CCR6 in IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCR6 fusion protein Ag24195
Full Name chemokine (C-C motif) receptor 6
Calculated Molecular Weight 42 kDa
GenBank Accession NumberBC037960
Gene Symbol CCR6
Gene ID (NCBI) 1235
RRIDAB_2882144
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR6, also known as CD196, is a C-C chemokine receptor that belongs to family A of G protein-coupled receptor superfamily. It is expressed in most B cells, memory T cells, and some subsets of dendritic cells (PMID: 17057190). CCR6 is a receptor for the C-C type chemokine CCL20 (PMID: 9169459). The ligand-receptor pair CCL20-CCR6 is responsible for the chemoattraction of immature dendritic cells, effector/memory T-cells, and B-cells and plays a role in skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and rheumatoid arthritis (PMID: 12948524). CCR6 polymorphism has been associated with rheumatoid arthritis susceptibility and CCR6 is involved in IL-17-driven autoimmunity in human diseases (PMID: 20453841).

Protocols

Product Specific Protocols
IHC protocol for CCR6 antibody 66801-1-IgDownload protocol
IF protocol for CCR6 antibody 66801-1-IgDownload protocol
FC protocol for CCR6 antibody 66801-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Neoplasia

Gut microbiota-derived short-chain fatty acids promote prostate cancer progression via inducing cancer cell autophagy and M2 macrophage polarization

Authors - Yufei Liu
View Article