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CoraLite® Plus 488 Anti-Mouse CD16 / CD32 (2.4G2)

CD16 / CD32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2.4G2

Cat no : CL488-65080

Synonyms

AI528646, CD16 / CD32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 488 Anti-Mouse CD16 / CD32 (2.4G2) (CL488-65080)

    FC experiment of mouse splenocytes using CL488-65080

    1X10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD16 / CD32 (CL488-65080, Clone:2.4G2) (red) or 0.25 ug Control Antibody. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65080 targets CD16 / CD32 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name Fc receptor, IgG, low affinity IIb
GenBank Accession NumberBC038070
Gene Symbol Fcgr2b
Gene ID (NCBI) 14130
RRIDAB_2883165
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD16 / CD32 antibody CL488-65080Download protocol
Standard Protocols
Click here to view our Standard Protocols