TEM1 Monoclonal antibody
TEM1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1F9B4
Cat no : 60170-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, COLO 320 cells, bEnd.3 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
60170-1-Ig targets TEM1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TEM1 fusion protein Ag13334 |
| Full Name | CD248 molecule, endosialin |
| Calculated Molecular Weight | 757 aa, 81 kDa |
| Observed Molecular Weight | 150-160 kDa |
| GenBank Accession Number | BC051340 |
| Gene Symbol | CD248 |
| Gene ID (NCBI) | 57124 |
| RRID | AB_10694834 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TEM1 antibody 60170-1-Ig | Download protocol |
| IHC protocol for TEM1 antibody 60170-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer | ||
Proc Natl Acad Sci U S A A membrane protein display platform for receptor interactome discovery. | ||
Oncotarget Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma. | ||
Sci Rep Tumor endothelial marker 1 is upregulated in heart after cardiac injury and participates in cardiac remodeling. | ||
Front Cardiovasc Med Soluble tumor endothelial marker 1 in heart failure with reduced ejection fraction: A pilot study | ||
EBioMedicine CD248 promotes insulin resistance by binding to the insulin receptor and dampening its insulin-induced autophosphorylation
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