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CD27 Polyclonal antibody

CD27 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 11121-1-AP

Synonyms

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CD27 Polyclonal antibody (11121-1-AP)

    WB analysis of Raji using 11121-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11121-1-AP (CD27 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Raji cells using CD27 Polyclonal antibody (11121-1-AP)

    FC experiment of Raji using 11121-1-AP

    1X10^6 Raji cells were stained with 0.2ug CD27 antibody (11121-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inRaji cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11121-1-AP targets CD27 in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD27 fusion protein Ag1582
Full Name CD27 molecule
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC012160
Gene Symbol CD27
Gene ID (NCBI) 939
RRIDAB_2073313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

Protocols

Product Specific Protocols
WB protocol for CD27 antibody 11121-1-APDownload protocol
FC protocol for CD27 antibody 11121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols