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APC Anti-Human CD45RA (HI100)

CD45RA Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

IF, FC

Conjugate

APC Fluorescent Dye

CloneNo.

HI100

Cat no : APC-65108

Synonyms

B220, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human peripheral blood mononuclear cells using APC Anti-Human CD45RA (HI100) (APC-65108)

    IF Staining of human peripheral blood mononuclear cells using APC-65108

    Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using APC Anti-Human CD45RA (APC-65108, Clone: HI100) and CoraLite®488-conjugated Anti-Human CD45RO (CL488-65150, Clone: UCHL1) at dilution of 1:300.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using APC Anti-Human CD45RA (HI100) (APC-65108)

    FC experiment of human peripheral blood lymphocytes using APC-65108

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.125 ug APC-Anti-Human CD45RA (APC-65108, clone HI100) (red) or 0.125 ug APC-mouse IgG2b isotype control (blue). Cells were not fixed.

Tested Applications

Positive IF detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:150-1:600
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65108 targets CD45RA in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC014239
Gene Symbol PTPRC
Gene ID (NCBI) 5788
RRIDAB_2882997
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
IF protocol for APC CD45RA antibody APC-65108Download protocol
FC protocol for APC CD45RA antibody APC-65108Download protocol
Standard Protocols
Click here to view our Standard Protocols