• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD62P (AK4)

CD62P Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

AK4

Cat no : 65164-1-Ig

Synonyms

AK4, CD62, CD62P, FLJ45155, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, SELP

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human peripheral blood platelets using Anti-Human CD62P (AK4) (65164-1-Ig)

    FC experiment of human peripheral blood platelets using 65164-1-Ig

    1X10^6 thrombin-activated human peripheral blood platelets were surface co-stained with Anti-Human CD41a labeled with FlexAble CL488 and 0.2 ug Anti-Human CD62P (65164-1-Ig, Clone:AK4) labeled with FlexAble CL647. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood platelets using Anti-Human CD62P (AK4) (65164-1-Ig)

    FC experiment of human peripheral blood platelets using 65164-1-Ig

    1X10^6 thrombin-activated human peripheral blood platelets were surface stained with 0.2 ug Anti-Human CD62P (65164-1-Ig, Clone:AK4) labeled with FlexAble CL647. Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65164-1-Ig targets CD62P in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Reactivity Human, Non-human primates
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human platelets
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Calculated Molecular Weight 830 aa, 91 kDa
GenBank Accession NumberBC028067
Gene Symbol SELP
Gene ID (NCBI) 6403
RRIDAB_2918443
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Protocols

Product Specific Protocols
FC protocol for CD62P antibody 65164-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols