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CoraLite® Plus 488-conjugated CD74 Monoclonal antibody

CD74 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D8A1

Cat no : CL488-66390

Synonyms

CD74, DHLAG, HLADG, Ia GAMMA, Ii, p33

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Raji cells using CoraLite® Plus 488-conjugated CD74 Monoclonal anti (CL488-66390)

    FC experiment of Raji using CL488-66390

    1X10^6 Raji cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD74 (CL488-66390, Clone:2D8A1) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66390 targets CD74 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD74 fusion protein Ag25503
Full Name CD74 molecule, major histocompatibility complex, class II invariant chain
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC018726
Gene Symbol CD74
Gene ID (NCBI) 972
RRIDAB_2883326
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD74 antibody CL488-66390Download protocol
Standard Protocols
Click here to view our Standard Protocols