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CD97 Polyclonal antibody

CD97 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 13071-1-AP

Synonyms

CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD97 Polyclonal antibody (13071-1-AP)

    WB analysis using 13071-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD97 Polyclonal antibody (13071-1-AP)

    WB analysis of Jurkat using 13071-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CD97 Polyclonal antibody (13071-1-AP)

    WB analysis of K-562 using 13071-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD97 Polyclonal antibody (13071-1-AP)

    WB analysis of U-937 using 13071-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD97 Polyclonal antibody (13071-1-AP)

    IHC staining of human tonsillitis using 13071-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD97 Polyclonal antibody (13071-1-AP)

    IHC staining of human tonsillitis using 13071-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD97 Polyclonal antibody (13071-1-AP)

    IHC staining of human lymphoma using 13071-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13071-1-AP (CD97 antibody) at dilution of 1:100 (under 10x lens).

  • Flow cytometry (FC) experiment of K-562 cells using CD97 Polyclonal antibody (13071-1-AP)

    FC experiment of K-562 using 13071-1-AP

    1X10^6 K-562 cells were stained with 0.2ug CD97 antibody (13071-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, U-937 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13071-1-AP targets CD97 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD97 fusion protein Ag3745
Full Name CD97 molecule
Calculated Molecular Weight 722 aa, 79 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
RRIDAB_10646477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

Protocols

Product Specific Protocols
WB protocol for CD97 antibody 13071-1-APDownload protocol
IHC protocol for CD97 antibody 13071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Fiona (Verified Customer) (01-09-2020)

Used sample to screen cell lines for expression of the CD97 protein. Didn't observe band where expected for U-937 cells (positive control) however assay requires further optimisation. Otherwise blot was clean. Specific clean staining observed when used for immunofluorescence.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Multiple cell lines