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CD99 Polyclonal antibody

CD99 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 23079-1-AP

Synonyms

12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD99 Polyclonal antibody (23079-1-AP)

    WB analysis using 23079-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23079-1-AP (CD99 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD99 Polyclonal antibody (23079-1-AP)

    WB analysis of U-937 using 23079-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 23079-1-AP (CD99 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CD99 Polyclonal antibody (23079-1-AP)

    IHC staining of human pancreas using 23079-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CD99 Polyclonal antibody (23079-1-AP)

    IHC staining of human pancreas using 23079-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CD99 Polyclonal antibody (23079-1-AP)

    IHC staining of human testis using 23079-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CD99 Polyclonal antibody (23079-1-AP)

    IHC staining of human testis using 23079-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of Jurkat cells using CD99 Polyclonal antibody (23079-1-AP)

    FC experiment of Jurkat using 23079-1-AP

    1X10^6 Jurkat cells were stained with 0.5ug CD99 antibody (23079-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inJurkat cells, U-937 cells, THP-1 cells
Positive IHC detected inhuman pancreas tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23079-1-AP is CD99 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD99 fusion protein Ag19461
Full Name CD99 molecule
Calculated Molecular Weight 19 kDa, 16 kDa, 17 kDa
Observed Molecular Weight 28 kDa, 32 kDa
GenBank Accession NumberBC021620
Gene Symbol CD99
Gene ID (NCBI) 4267
RRIDAB_2879205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).

Protocols

Product Specific Protocols
WB protocol for CD99 antibody 23079-1-APDownload protocol
IHC protocol for CD99 antibody 23079-1-APDownload protocol
FC protocol for CD99 antibody 23079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIHC,IF

J Anim Sci Biotechnol

Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis.

Authors - Lingkai Zhang
View Article
humanIHC

Oncol Lett

Metastatic extraskeletal Ewing's sarcoma treated with trabectedin: A case report.

Authors - Jorge Hernando-Cubero
View Article
humanIHC

Cell Death Dis

KDM5B promotes tumorigenesis of Ewing sarcoma via FBXW7/CCNE1 axis.

Authors - Binbin Chen
View Article