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Cadherin-11 Polyclonal antibody

Cadherin-11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12028-1-AP

Synonyms

CAD11, Cadherin 11, Cadherin-11, CDH11, CDHOB, OB, OB cadherin, OSF 4, Osteoblast cadherin

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using Cadherin-11 Polyclonal antibody (12028-1-AP)

    WB analysis of PC-3 using 12028-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12028-1-AP (Cadherin-11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12028-1-AP targets Cadherin-11 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cadherin-11 fusion protein Ag2659
Full Name cadherin 11, type 2, OB-cadherin (osteoblast)
Calculated Molecular Weight 796 aa, 88 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC013609
Gene Symbol CDH11
Gene ID (NCBI) 1009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-11 (CDH11), also known osteoblast cadherin (OB-cadherin) or OSF-4, was identified initially in a mouse osteoblastic cell line and human osteosarcoma (PMID: 8163513). Cadherin-11 is a single pass transmembrane protein consisting of an extracellular domain which contains five repeat sequences (EC1-EC5), a transmembrane segment and a cytoplasmic domain which links catenins to the actin and microtubule cytoskeleton (PMID: 8163513; 33442417). It has been shown that cadherin-11 plays important roles in the occurrence and development of a lot of diseases, arthritis, cancer, aortic calcification disease, dermatosis (PMID: 33442417).

Protocols

Product Specific Protocols
WB protocol for Cadherin-11 antibody 12028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols