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Cadherin-18 Polyclonal antibody

Cadherin-18 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13091-1-AP

Synonyms

cadherin 18, type 2, Cadherin-14, Cadherin-18, CDH14, CDH14L, CDH18, EY CADHERIN

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using Cadherin-18 Polyclonal antibody (13091-1-AP)

    WB analysis of COLO 320 using 13091-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13091-1-AP (Cadherin-18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Cadherin-18 Polyclonal antibody (13091-1-AP)

    WB analysis of A549 using 13091-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13091-1-AP (Cadherin-18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Cadherin-18 Polyclonal antibody (13091-1-AP)

    IHC staining of human gliomas using 13091-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Cadherin-18 Polyclonal antibody (13091-1-AP)

    IHC staining of human gliomas using 13091-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Cadherin-18 Polyclonal antibody (13091-1-AP)

    IHC staining of rat cerebellum using 13091-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Cadherin-18 Polyclonal antibody (13091-1-AP)

    IHC staining of rat cerebellum using 13091-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells, A549 cells
Positive IHC detected inhuman gliomas tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13091-1-AP targets Cadherin-18 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cadherin-18 fusion protein Ag3675
Full Name cadherin 18, type 2
Calculated Molecular Weight 790 aa, 88 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC031051
Gene Symbol CDH18
Gene ID (NCBI) 1016
RRIDAB_10646435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Cadherin-18 antibody 13091-1-APDownload protocol
IHC protocol for Cadherin-18 antibody 13091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

NPJ Regen Med

CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cells.

Authors - Julia Junghof
View Article
humanWB,IHC

Cell Physiol Biochem

A Novel Tumor-Suppressor, CDH18, Inhibits Glioma Cell Invasiveness Via UQCRC2 and Correlates with the Prognosis of Glioma Patients.

Authors - Ya-Hui Bai
View Article