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Cadherin-20 Polyclonal antibody

Cadherin-20 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22049-1-AP

Synonyms

Cadherin 20, cadherin 20, type 2, Cadherin-20, CDH20, Cdh7, CDH7L3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cadherin-20 Polyclonal antibody (22049-1-AP)

    WB analysis using 22049-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 22049-1-AP (Cadherin-20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cadherin-20 Polyclonal antibody (22049-1-AP)

    WB analysis of HeLa using 22049-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22049-1-AP (Cadherin-20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Cadherin-20 Polyclonal antibody (22049-1-AP)

    WB analysis of mouse brain using 22049-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22049-1-AP (Cadherin-20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cadherin-20 Polyclonal antibody (22049-1-AP)

    IHC staining of human colon using 22049-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cadherin-20 Polyclonal antibody (22049-1-AP)

    IHC staining of human colon using 22049-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Cadherin-20 Polyclonal antibody (22049-1-AP)

    IHC staining of mouse brain using 22049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Cadherin-20 Polyclonal antibody (22049-1-AP)

    IHC staining of mouse brain using 22049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Cadherin-20 Polyclonal antibody (22049-1-AP)

    IHC staining of rat brain using 22049-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Cadherin-20 Polyclonal antibody (22049-1-AP)

    IHC staining of rat brain using 22049-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22049-1-AP (Cadherin-20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman placenta tissue, HeLa cells, mouse brain tissue
Positive IHC detected inhuman colon tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22049-1-AP is Cadherin-20 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cadherin-20 fusion protein Ag16990
Full Name cadherin 20, type 2
Calculated Molecular Weight 801 aa, 89 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC101048
Gene Symbol CDH20
Gene ID (NCBI) 28316
RRIDAB_11125960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Cadherin-20 antibody 22049-1-APDownload protocol
IHC protocol for Cadherin-20 antibody 22049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols