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CDKN2AIP Polyclonal antibody

CDKN2AIP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16615-1-AP

Synonyms

CARF, CDKN2A interacting protein, CDKN2AIP, Collaborator of ARF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDKN2AIP Polyclonal antibody (16615-1-AP)

    WB analysis using 16615-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CDKN2AIP Polyclonal antibody (16615-1-AP)

    WB analysis of Jurkat using 16615-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDKN2AIP Polyclonal antibody (16615-1-AP)

    WB analysis of HeLa using 16615-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CDKN2AIP Polyclonal antibody (16615-1-AP)

    WB analysis of Jurkat using 16615-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IP experiment of HeLa using 16615-1-AP

    IP result of anti-CDKN2AIP (IP:16615-1-AP, 4ug; Detection:16615-1-AP 1:500) with HeLa cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human kidney using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human placenta using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human testis using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human testis using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human skin using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human skin using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human liver using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human liver using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human spleen using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human spleen using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human ovary using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human ovary using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human placenta using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IHC staining of human kidney using 16615-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDKN2AIP Polyclonal antibody (16615-1-AP)

    IF Staining of HepG2 using 16615-1-AP

    Immunofluorescent analysis of HepG2 cells, using 16615-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inCOLO 320 cells, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16615-1-AP targets CDKN2AIP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDKN2AIP fusion protein Ag9907
Full Name CDKN2A interacting protein
Calculated Molecular Weight 579 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC022270
Gene Symbol CDKN2AIP
Gene ID (NCBI) 55602
RRIDAB_2244775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CDKN2AIP antibody 16615-1-APDownload protocol
IHC protocol for CDKN2AIP antibody 16615-1-APDownload protocol
IF protocol for CDKN2AIP antibody 16615-1-APDownload protocol
IP protocol for CDKN2AIP antibody 16615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IF

Cell Biosci

CDKN2AIP is critical for spermiogenesis and germ cell development

Authors - Yuming Cao
View Article
humanWB

Clin Cosmet Investig Dermatol

Genome Wide Differential Expression Profiles in Nevus Sebaceous Uncovered Low Expression of CDKN2AIP and Construction of a ceRNA Network.

Authors - Xianhong Yang
View Article