• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CDYL Polyclonal antibody

CDYL Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17763-1-AP

Synonyms

CDY like, CDYL, CDYL1, chromodomain protein, Y like, Chromodomain Y like protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of PC-3 cells using CDYL Polyclonal antibody (17763-1-AP)

    WB analysis of PC-3 using 17763-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CDYL Polyclonal antibody (17763-1-AP)

    WB analysis of mouse testis using 17763-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CDYL Polyclonal antibody (17763-1-AP)

    WB analysis of MCF-7 using 17763-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using CDYL Polyclonal antibody (17763-1-AP)

    IP experiment of MCF-7 using 17763-1-AP

    IP result of anti-CDYL (IP:17763-1-AP, 4ug; Detection:17763-1-AP 1:1000) with MCF-7 cells lysate 3600 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDYL Polyclonal antibody (17763-1-AP)

    IHC staining of human breast cancer using 17763-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDYL Polyclonal antibody (17763-1-AP)

    IHC staining of human breast cancer using 17763-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDYL Polyclonal antibody (17763-1-AP)

    IHC staining of human lung cancer using 17763-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDYL Polyclonal antibody (17763-1-AP)

    IHC staining of human lung cancer using 17763-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-3 cells, mouse testis tissue, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17763-1-AP targets CDYL in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDYL fusion protein Ag12143
Full Name chromodomain protein, Y-like
Calculated Molecular Weight 544 aa, 61 kDa
Observed Molecular Weight 66 kDa, 60 kDa
GenBank Accession NumberBC108725
Gene Symbol CDYL
Gene ID (NCBI) 9425
RRIDAB_2077197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CDYL antibody 17763-1-APDownload protocol
IHC protocol for CDYL antibody 17763-1-APDownload protocol
IP protocol for CDYL antibody 17763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Endocrinology

Dynamic profiles and transcriptional preferences of histone modifications during spermiogenesis.

Authors - Yinchuan Li
View Article
mouseWB

Adv Sci (Weinh)

Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons.

Authors - Zhao-Wei Sun
View Article
ratWB

Drug Des Devel Ther

Sorafenib Attenuates Fibrotic Hepatic Injury Through Mediating Lysine Crotonylation

Authors - Xiao-Feng Chen
View Article
humanWB

Cancer Lett

CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment

Authors - Jin-Ge Kong
  • KD Validated
View Article