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CEA Monoclonal antibody

CEA Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

3D1A6

Cat no : 60053-1-Ig

Synonyms

Carcinoembryonic antigen, CD66e, CEA, CEACAM5, Meconium antigen 100

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using CEA Monoclonal antibody (60053-1-Ig)

    WB analysis of human colon using 60053-1-Ig

    human colon tissue were subjected to SDS PAGE followed by western blot with 60053-1-Ig (CEA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60053-1-Ig targets CEA in ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CEA fusion protein Ag0672
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Calculated Molecular Weight 77 kDa
GenBank Accession NumberBC034671
Gene Symbol CEACAM5
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic Acid/Ammonium Sulfate Precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.