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CEBPE Polyclonal antibody

CEBPE Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 14271-1-AP

Synonyms

C/EBP epsilon, CEBPE, CRP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using CEBPE Polyclonal antibody (14271-1-AP)

    WB analysis of mouse liver using 14271-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14271-1-AP (CEBPE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CEBPE Polyclonal antibody (14271-1-AP)

    WB analysis of U-937 using 14271-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14271-1-AP (CEBPE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEBPE Polyclonal antibody (14271-1-AP)

    IF Staining of HepG2 using 14271-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14271-1-AP (CEBPE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, U-937 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14271-1-AP targets CEBPE in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEBPE fusion protein Ag5589
Full Name CCAAT/enhancer binding protein (C/EBP), epsilon
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC035797
Gene Symbol CEBPE
Gene ID (NCBI) 1053
RRIDAB_2878038
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CEBPE antibody 14271-1-APDownload protocol
IF protocol for CEBPE antibody 14271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Ann N Y Acad Sci

LncRNA-ES3 inhibition by Bhlhe40 is involved in high glucose-induced calcification/senescence of vascular smooth muscle cells.

Authors - Jia-Yu Zhong
View Article