CEBPZ Polyclonal antibody

CEBPZ Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25612-1-AP

Synonyms

CBF, CBF2, CCAAT binding factor, CEBPZ, HSP CBF, NOC1



Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, Jurkat cells, K-562 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

25612-1-AP targets CEBPZ in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEBPZ fusion protein Ag11003
Full Name CCAAT/enhancer binding protein (C/EBP), zeta
Calculated Molecular Weight 1054 aa, 121 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC034475
Gene Symbol CEBPZ
Gene ID (NCBI) 10153
RRIDAB_2880160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEBPZ (CCAAT/enhancer binding protein zeta) belongs to the CBF/Mak21 family, it plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner.

Protocols

Product Specific Protocols
WB protocol for CEBPZ antibody 25612-1-APDownload protocol
IF protocol for CEBPZ antibody 25612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Sci

CEBPγ facilitates lamellipodia formation and cancer cell migration through CERS6 upregulation.

Authors - Hanxiao Shi
mouseWB

Mol Cell Proteomics

Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression.

Authors - Tong Wu