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CEND1 Polyclonal antibody

CEND1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13280-1-AP

Synonyms

BM88, BM88 antigen, CEND1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEND1 Polyclonal antibody (13280-1-AP)

    WB analysis using 13280-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CEND1 Polyclonal antibody (13280-1-AP)

    WB analysis using 13280-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CEND1 Polyclonal antibody (13280-1-AP)

    WB analysis of human brain using 13280-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CEND1 Polyclonal antibody (13280-1-AP)

    IHC staining of mouse brain using 13280-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using CEND1 Polyclonal antibody (13280-1-AP)

    IHC staining of rat brain using 13280-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using CEND1 Polyclonal antibody (13280-1-AP)

    IF Staining of Neuro-2a using 13280-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CEND1 antibody (13280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13280-1-AP targets CEND1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEND1 fusion protein Ag4024
Full Name cell cycle exit and neuronal differentiation 1
Calculated Molecular Weight 149 aa, 15 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC034732
Gene Symbol CEND1
Gene ID (NCBI) 51286
RRIDAB_2291632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell cycle exit and neuronal differentiation 1 (CEND1) also known as BM88, is a neuronal protein associated in vivo with terminal neuron-generating divisions, marking the exit of proliferative cells from the cell cycle (PMID: 17971443). CEND1 expression is critical for the NEUROG2-driven reprogramming of astrocytes, suggesting the existence of a reciprocal feedback loop leading to neurogenesis (PMID: 26321141). CEND1 is an integral membrane protein composed of two 23-kDa polypeptide chains linked together by disulfide bridges.

Protocols

Product Specific Protocols
WB protocol for CEND1 antibody 13280-1-APDownload protocol
IHC protocol for CEND1 antibody 13280-1-APDownload protocol
IF protocol for CEND1 antibody 13280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols