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CENPH Polyclonal antibody

CENPH Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12841-1-AP

Synonyms

CENP H, CENPH, centromere protein H, ICEN35, NNF1, PMF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CENPH Polyclonal antibody (12841-1-AP)

    WB analysis using 12841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12841-1-AP (CENPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CENPH Polyclonal antibody (12841-1-AP)

    IP experiment of HeLa using 12841-1-AP

    IP result of anti-CENPH (IP:12841-1-AP, 4ug; Detection:12841-1-AP 1:1000) with HeLa cells lysate 1600 ug.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12841-1-AP targets CENPH in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPH fusion protein Ag3836
Full Name centromere protein H
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC015355
Gene Symbol CENPH
Gene ID (NCBI) 64946
RRIDAB_2076634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Centromere protein H (CENPH) is a component of the active centromere complex that associates with centromeric DNA and binds spindle microtubules to the chromosomes, which is required for chromosome congression and efficiently align the chromosomes on a metaphase plate. CENPH plays a key role in assembly of kinetochore proteins, mitotic progression and chromosome segregation that may cause misaligned chromosomes and multipolar spindles. Besides, CENPH may be associated with the modulation of the cell cycle through an interaction with CENPC. It has been shown that the expression of CENPH is related to clinical application including colorectal cancer, gastric cancer, renal cell carcinoma, hepatocellular carcinoma and other poor prognosis in various solid tumors. (PMID: 26378239, 27993978, 28819418)

Protocols

Product Specific Protocols
WB protocol for CENPH antibody 12841-1-APDownload protocol
IP protocol for CENPH antibody 12841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuro Oncol

Mitogen-Induced Defective Mitosis Transforms Neural Progenitor Cells

Authors - Hiba K Omairi
View Article
humanWB

Am J Cancer Res

CENPH overexpression promotes the progression, cisplatin resistance, and poor prognosis of lung adenocarcinoma via the AKT and ERK/P38 pathways

Authors - Qiao Li
  • KD Validated
View Article