CFTR Monoclonal antibody

CFTR Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F4A8

Cat no : 66928-1-Ig

Synonyms

ABC35, ABCC7, CF, CFTR, CFTR/MRP, dJ760C5.1, MRP7, TNR CFTR



Tested Applications

Positive WB detected inCalu-3 cells, HeLa cells, A431 cells, A375 cells, A549 cells, NCI-H1299 cells, HepG2 cells, MCF-7 cells, HT-29 cells, Caco-2 cells, MOLT-4 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66928-1-Ig targets CFTR in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CFTR fusion protein Ag27810
Full Name cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Calculated Molecular Weight 168 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberNM_000492
Gene Symbol CFTR
Gene ID (NCBI) 1080
RRIDAB_2882254
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CFTR is a member of the ATP-binding cassette (ABC) family of membrane transport proteins, functioning as a chloride channel responsible for ion flow across epithelial surfaces of lung, sinuses, pancreas, intestine, and liver. Mutations of CFTR cause cystic fibrosis (CF), a disorder affecting the respiratory, digestive, reproductive systems and sweat glands.

Protocols

Product Specific Protocols
WB protocol for CFTR antibody 66928-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols