CHD4 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66222-1-Ig |CloneNo.: 3D9G2

Featured Product KD/KO validated

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Freight/Packing

Con: 150 μg/150 μl

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Species specificity:
human

Positive WB detected in:
MCF-7 cells,HEK-293 cells

Positive IHC detected in:
human cervical cancer tissue

Positive IF detected in:
HEK-293 cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
chromodomain helicase DNA binding protein 4

Calculated molecular weight:
1937aa,221 kDa

Observed molecular weight:
260 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CHD4

Synonyms
ATP dependent helicase CHD4, CHD 4, CHD4, DKFZp686E06161, Mi 2b, Mi2 BETA
Background

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.


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