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  • Featured Product
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CHD4 Monoclonal antibody

CHD4 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D9G2

Cat no : 66222-1-Ig

Synonyms

ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    WB analysis of HEK-293 using 66222-1-Ig

    WB result of CHD4 antibody (66222-1-Ig, 1:1000) with si-Control and si-CHD4 transfected HEK293 cells.

  • Western Blot (WB) analysis of MCF-7 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    WB analysis of MCF-7 using 66222-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    WB analysis of HEK-293 using 66222-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    WB analysis of HEK-293 using 66222-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CHD4 Monoclonal antibody (66222-1-Ig)

    IHC staining of human cervical cancer using 66222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66222-1-Ig (CHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CHD4 Monoclonal antibody (66222-1-Ig)

    IHC staining of human cervical cancer using 66222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66222-1-Ig (CHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CHD4 Monoclonal antibody (66222-1-Ig)

    IF Staining of HEK-293 using 66222-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HEK-293 cells using 66222-1-Ig(CHD4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66222-1-Ig targets CHD4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHD4 fusion protein Ag5729
Full Name chromodomain helicase DNA binding protein 4
Calculated Molecular Weight 1937 aa, 221 kDa
Observed Molecular Weight 260 kDa
GenBank Accession NumberBC038596
Gene Symbol CHD4
Gene ID (NCBI) 1108
RRIDAB_2881613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.

Protocols

Product Specific Protocols
WB protocol for CHD4 antibody 66222-1-IgDownload protocol
IHC protocol for CHD4 antibody 66222-1-IgDownload protocol
IF protocol for CHD4 antibody 66222-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai
View Article