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CHIA Polyclonal antibody

CHIA Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21484-1-AP

Synonyms

2200003E03Rik, Acidic mammalian chitinase, AMCase, CHIA, CHIT2, chitinase, acidic, Lung specific protein TSA1902, RP5 1125M8.1, TSA1902

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CHIA Polyclonal antibody (21484-1-AP)

    WB analysis of mouse lung using 21484-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21484-1-AP (CHIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CHIA Polyclonal antibody (21484-1-AP)

    WB analysis of mouse lung using 21484-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21484-1-AP (CHIA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using CHIA Polyclonal antibody (21484-1-AP)

    IHC staining of human stomach using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CHIA Polyclonal antibody (21484-1-AP)

    IHC staining of human stomach using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CHIA Polyclonal antibody (21484-1-AP)

    IHC staining of human lung using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CHIA Polyclonal antibody (21484-1-AP)

    IHC staining of human lung using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHIA Polyclonal antibody (21484-1-AP)

    IF Staining of HepG2 using 21484-1-AP

    Immunofluorescent analysis of HepG2 cells, using CHIA antibody 21484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CHIA Polyclonal antibody (21484-1-AP)

    IF Staining of HeLa using 21484-1-AP

    Immunofluorescent analysis of Hela cells, using CHIA antibody 21484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman stomach tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21484-1-AP is CHIA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHIA fusion protein Ag15767
Full Name chitinase, acidic
Calculated Molecular Weight 476 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC036339
Gene Symbol CHIA
Gene ID (NCBI) 27159
RRIDAB_10755301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHIA antibody 21484-1-APDownload protocol
IHC protocol for CHIA antibody 21484-1-APDownload protocol
IF protocol for CHIA antibody 21484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Mol Biosci

Characterization of an Aging-Based Diagnostic Gene Signature and Molecular Subtypes With Diverse Immune Infiltrations in Atherosclerosis.

Authors - Lei Zhao
View Article
mouseIF

Commun Biol

Elevated stress response marks deeply quiescent reserve cells of gastric chief cells

Authors - Daisuke Shiokawa
View Article
mouseWB,IF

Eur J Immunol

Aspergillus fumigatus corneal infection is regulated by chitin synthases and by neutrophil-derived acidic mammalian chitinase.

Authors - Steven de Jesus Carrion
View Article