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  • KD/KO Validated

Choline Kinase Alpha Polyclonal antibody

Choline Kinase Alpha Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13520-1-AP

Synonyms

CHETK alpha, CHK, CHKA, Choline kinase alpha, CK, CKI, CK-α, EK, Ethanolamine kinase;



Tested Applications

Positive WB detected inmouse spleen tissue, COLO 320 cells, mouse colon tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13520-1-AP targets Choline Kinase Alpha in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Choline Kinase Alpha fusion protein Ag4761
Full Name choline kinase alpha
Calculated Molecular Weight 439 aa, 52 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC036471
Gene Symbol CHKA
Gene ID (NCBI) 1119
RRIDAB_2079160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline phosphotransferase) is a cytosolic enzyme that catalyzes the committed step in the synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the phosphorylation of choline with ATP to form phosphocholine and ADP(PMID:9506987). It has 2 isoforms produced by alternative splicing with the molecular weight of 52 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for Choline Kinase Alpha antibody 13520-1-APDownload protocol
IHC protocol for Choline Kinase Alpha antibody 13520-1-APDownload protocol
IF protocol for Choline Kinase Alpha antibody 13520-1-APDownload protocol
IP protocol for Choline Kinase Alpha antibody 13520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Proc Natl Acad Sci U S A

The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation

Authors - Qingxia Ma
mouseWB, IHC

Ann Rheum Dis

Choline kinase inhibition in rheumatoid arthritis.

Authors - M Guma
humanWB

Clin Cancer Res

Preclinical development of a non-toxic oral formulation of monoethanolamine, a lipid precursor, for prostate cancer treatment.

Authors - Roopali Saxena
mouseWB

Front Cell Dev Biol

Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration.

Authors - Ming Kong
ratWB

J Biol Chem

ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.

Authors - Arsenault Daniel J DJ
WB

Mol Biol Cell

Nuclear-localized CTP:phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis.

Authors - Adam J Aitchison

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (02-02-2021)

I was surprised it did not work as shown in the datasheet. It is possible jus this batch?

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse liver and mouse spleen
Choline Kinase Alpha Antibody Western Blot validation (1:500 dilution) in mouse liver and mouse spleen (Cat no:13520-1-AP)