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CHMP6 Polyclonal antibody

CHMP6 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 16278-1-AP

Synonyms

CHMP6, chromatin modifying protein 6, hVps20, VPS20

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using CHMP6 Polyclonal antibody (16278-1-AP)

    WB analysis of mouse testis using 16278-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CHMP6 Polyclonal antibody (16278-1-AP)

    WB analysis of Jurkat using 16278-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CHMP6 Polyclonal antibody (16278-1-AP)

    WB analysis of U-937 using 16278-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using CHMP6 Polyclonal antibody (16278-1-AP)

    WB analysis of A375 using 16278-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CHMP6 Polyclonal antibody (16278-1-AP)

    WB analysis of A431 using 16278-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CHMP6 Polyclonal antibody (16278-1-AP)

    IP experiment of mouse testis using 16278-1-AP

    IP result of anti-CHMP6 (IP:16278-1-AP, 4ug; Detection:16278-1-AP 1:500) with mouse testis tissue lysate 2400ug.

Tested Applications

Positive WB detected inmouse testis tissue, A375 cells, A431 cells, Jurkat cells, U-937 cells
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16278-1-AP targets CHMP6 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP6 fusion protein Ag9357
Full Name chromatin modifying protein 6
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC010108
Gene Symbol CHMP6
Gene ID (NCBI) 79643
RRIDAB_2079498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHMP6 antibody 16278-1-APDownload protocol
IP protocol for CHMP6 antibody 16278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
View Article
canineWB

Curr Biol

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

Authors - Eva P Karasmanis
View Article
humanWB

J Virol

A non-envelopment role for the ESCRT-III complex during HCMV infection.

Authors - Nicholas T Streck
View Article
humanWB

J Virol

Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread.

Authors - Nicholas T Streck
View Article
humanWB,IF

Nat Commun

Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy

Authors - Takashi Nozawa
  • KD Validated
View Article