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CHN1 Polyclonal antibody

CHN1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12048-1-AP

Synonyms

A chimaerin, Alpha chimerin, ARHGAP2, chimerin (chimaerin) 1, CHN, CHN1, DURS2, N chimaerin, N chimerin, NC, RHOGAP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHN1 Polyclonal antibody (12048-1-AP)

    WB analysis using 12048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12048-1-AP (CHN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CHN1 Polyclonal antibody (12048-1-AP)

    IHC staining of human gliomas using 12048-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CHN1 Polyclonal antibody (12048-1-AP)

    IHC staining of human gliomas using 12048-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHN1 Polyclonal antibody (12048-1-AP)

    IHC staining of mouse brain using 12048-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHN1 Polyclonal antibody (12048-1-AP)

    IHC staining of mouse brain using 12048-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHN1 Polyclonal antibody (12048-1-AP)

    IHC staining of mouse brain using 12048-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHN1 Polyclonal antibody (12048-1-AP)

    IHC staining of mouse brain using 12048-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CHN1 Polyclonal antibody (12048-1-AP)

    IHC staining of human gliomas using 12048-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CHN1 Polyclonal antibody (12048-1-AP)

    IHC staining of human gliomas using 12048-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, PC-12 cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12048-1-AP targets CHN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHN1 fusion protein Ag2678
Full Name chimerin (chimaerin) 1
Calculated Molecular Weight 459 aa, 53 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC011393
Gene Symbol CHN1
Gene ID (NCBI) 1123
RRIDAB_10794446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHN1 antibody 12048-1-APDownload protocol
IHC protocol for CHN1 antibody 12048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis.

Authors - Jianbing Liu
View Article