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  • Featured Product
  • KD/KO Validated

CHRNB4 Polyclonal antibody

CHRNB4 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 22192-1-AP

Synonyms

CHRNB4

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using CHRNB4 Polyclonal antibody (22192-1-AP)

    WB analysis of SH-SY5Y using 22192-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CHRNB4 Polyclonal antibody (22192-1-AP)

    WB analysis of HeLa using 22192-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CHRNB4 Polyclonal antibody (22192-1-AP)

    WB analysis of mouse testis using 22192-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CHRNB4 Polyclonal antibody (22192-1-AP)

    FC experiment of SH-SY5Y using 22192-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug CHRNB4 antibody (22192-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells, mouse testis tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22192-1-AP is CHRNB4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNB4 fusion protein Ag16767
Full Name cholinergic receptor, nicotinic, beta 4
Calculated Molecular Weight 498 aa, 56 kDa
Observed Molecular Weight 48-60 kDa
GenBank Accession NumberBC096080
Gene Symbol CHRNB4
Gene ID (NCBI) 1143
RRIDAB_11232227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHRNB4 antibody 22192-1-APDownload protocol
FC protocol for CHRNB4 antibody 22192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Mar Drugs

Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.

Authors - Zhihua Sun
View Article
humanWB

Eur J Pharmacol

Identification of nicotinic acetylcholine receptor subunits in different lung cancer cell lines and the inhibitory effect of alpha-conotoxin TxID on lung cancer cell growth.

Authors - Jiang Qian
View Article
humanWB

Cancers (Basel)

An Integrated Genomic Strategy to Identify CHRNB4 as a Diagnostic/Prognostic Biomarker for Targeted Therapy in Head and Neck Cancer.

Authors - Yi-Hsuan Chuang
  • KO Validated
View Article
humanWB

Mar Drugs

Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets.

Authors - Yiqiao Liu
View Article