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CHST10 Polyclonal antibody

CHST10 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12013-1-AP

Synonyms

CHST10, HNK 1 sulfotransferase, HNK 1ST, HNK1ST, HuHNK 1ST

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using CHST10 Polyclonal antibody (12013-1-AP)

    WB analysis of BxPC-3 using 12013-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12013-1-AP (CHST10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CHST10 Polyclonal antibody (12013-1-AP)

    IHC staining of human gliomas using 12013-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12013-1-AP (CHST10 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12013-1-AP targets CHST10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHST10 fusion protein Ag2627
Full Name carbohydrate sulfotransferase 10
Calculated Molecular Weight 356 aa, 42 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC010441
Gene Symbol CHST10
Gene ID (NCBI) 9486
RRIDAB_2229573
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHST10 antibody 12013-1-APDownload protocol
IHC protocol for CHST10 antibody 12013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols