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CHST8 Polyclonal antibody

CHST8 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16426-1-AP

Synonyms

CHST8, GalNAc 4 O sulfotransferase 1, GalNAc 4 ST1, GalNAc4ST, GalNAc4ST 1, GALNAC4ST1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using CHST8 Polyclonal antibody (16426-1-AP)

    WB analysis of mouse heart using 16426-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16426-1-AP (CHST8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using CHST8 Polyclonal antibody (16426-1-AP)

    IF Staining of mouse skin using 16426-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CHST8 antibody (16426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using CHST8 Polyclonal antibody (16426-1-AP)

    IF Staining of mouse skin using 16426-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CHST8 antibody (16426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse heart tissue
Positive IF detected inmouse skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16426-1-AP targets CHST8 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHST8 fusion protein Ag9669
Full Name carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8
Calculated Molecular Weight 424 aa, 49 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC014250
Gene Symbol CHST8
Gene ID (NCBI) 64377
RRIDAB_2229376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHST8 antibody 16426-1-APDownload protocol
IF protocol for CHST8 antibody 16426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols