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CIRBP Monoclonal antibody

CIRBP Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4D12H7

Cat no : 60025-2-Ig

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CIRBP Monoclonal antibody (60025-2-Ig)

    WB analysis of HEK-293 using 60025-2-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60025-2-Ig (CIRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CIRBP Monoclonal antibody (60025-2-Ig)

    IHC staining of human tonsillitis using 60025-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 60025-2-Ig (CIRBP Antibody) at dilution of 1:50.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

60025-2-Ig targets CIRBP in IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen CIRBP fusion protein Ag0280
Full Name cold inducible RNA binding protein
Calculated Molecular Weight 172 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC000403
Gene Symbol CIRBP
Gene ID (NCBI) 1153
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation. It acts as a translational repressor. CIRBP is an immortalized gene in murine primary cells. CIRBP promotes assembly of stress granules (SGs), when overexpressed. It is a potential proto-oncogenic proteins upon overexpression.(PMID:19900510) Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature. (PMID:20735994) This antibody can bind the close sequences genes. This antibody is a mouse monoclonal antibody raised against full length CIRBP of human origin.

Publications

SpeciesApplicationTitle
humanIHC

Ann Dermatol

Expression of Cold-Inducible RNA-Binding Protein in Normal Skin, Actinic Keratosis and Squamous Cell Carcinoma.

Authors - Bo Mi Park
View Article