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CITED1 Polyclonal antibody

CITED1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26999-1-AP

Synonyms

CITED1, Melanocyte specific protein 1, MSG1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat testis tissue using CITED1 Polyclonal antibody (26999-1-AP)

    WB analysis of rat testis using 26999-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CITED1 Polyclonal antibody (26999-1-AP)

    WB analysis of mouse testis using 26999-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using CITED1 Polyclonal antibody (26999-1-AP)

    WB analysis of A375 using 26999-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A375 cells using CITED1 Polyclonal antibody (26999-1-AP)

    IP experiment of A375 using 26999-1-AP

    IP result of anti-CITED1 (IP:26999-1-AP, 4ug; Detection:26999-1-AP 1:300) with A375 cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CITED1 Polyclonal antibody (26999-1-AP)

    IHC staining of human colon cancer using 26999-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CITED1 Polyclonal antibody (26999-1-AP)

    IHC staining of human colon cancer using 26999-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using CITED1 Polyclonal antibody (26999-1-AP)

    IHC staining of rat testis using 26999-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using CITED1 Polyclonal antibody (26999-1-AP)

    IHC staining of rat testis using 26999-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CITED1 Polyclonal antibody (26999-1-AP)

    IHC staining of human thyroid cancer using 26999-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CITED1 Polyclonal antibody (26999-1-AP)

    IHC staining of human thyroid cancer using 26999-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CITED1 Polyclonal antibody (26999-1-AP)

    IF Staining of HEK-293 using 26999-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CITED1 antibody (26999-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293T cells using CITED1 Polyclonal antibody (26999-1-AP)

    FC experiment of HEK-293T using 26999-1-AP

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human CITED1 (26999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inrat testis tissue, A375 cells, mouse testis tissue
Positive IP detected inA375 cells
Positive IHC detected inhuman colon cancer tissue, human thyroid cancer tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26999-1-AP is CITED1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CITED1 fusion protein Ag24643
Full Name Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC004240
Gene Symbol CITED1
Gene ID (NCBI) 4435
RRIDAB_2880718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. The CITED1 gene encodes a 27 kDa protein, but a 30 kDa band was also detected in the publication. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526, 15272279, 15843474).

Protocols

Product Specific Protocols
WB protocol for CITED1 antibody 26999-1-APDownload protocol
IHC protocol for CITED1 antibody 26999-1-APDownload protocol
IF protocol for CITED1 antibody 26999-1-APDownload protocol
IP protocol for CITED1 antibody 26999-1-APDownload protocol
FC protocol for CITED1 antibody 26999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation

Authors - Xin Gu
View Article